FlexSTAR

The FlexSTAR workflow, which extracts DNA from 0.5-10 ml of sample/protocols (i.e. whole blood), combines our robust and reliable instrumentation with Qiagen’s proven Flexigene chemistry. Our instrument, which is fully automated and easy-to-use with no manual intervention required, can isolate up to 30 samples per run – consistently producing high-quality, high-yield genomic DNA ready for your next application or long-term storage by biorepositories. The FlexSTAR offers a sample tracking and reporting package that utilizes bar code identification of samples to preserve sample integrity.

Principle & Procedure

1. Lysis buffer is added to the sample (e.g. blood) and cell nuclei and mitochondria are pelleted by centrifugation
2. The pellet is resuspended and incubated in denaturation buffer containing a chaotropic salt and QIAGEN Protease – efficiently removing contaminants (e.g. proteins)
3. DNA is precipitated by the addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, dried, and resuspended in hydration buffer (10mM Tris·Cl, pH 8.5)

Instrument Features

• Uses Qiagen’s proven precipitation chemistry for high molecular weight genomic DNA, free of RNA contamination
• Produces high-yield, high-quality DNA ready for immediate use or long-term storage
• Features protocols for 0.5-2 ml, 3-5ml, and 6-10ml of fresh or frozen whole blood
• Includes our Oragene®  and Isohelix Saliva protocols allowing labs to process up to 30 4ml saliva tubes at a time
• Process culture cell samples, up to 30 samples of 80 million cells each
• Plus protocols for buffy coats derived from 10ml (30 at a time) or 20ml (15 at a time)
• Runs up to 30 samples in a single batch
• Has an internal barcode scanner for positive sample tracking to verify sample input and DNA output positions

Tracking & Reporting Features

• Compiles run reports and communicates directly to LIMs system
• Creates maps of samples and DNA output positions
• Tracks samples through the extraction process
• Sends emails or text alerts when the run is complete

Sample Type/Protocols

• Cells
• PBMCs
• Oragene DNA saliva
• Fresh/frozen buffy coats
• Buccal swabs
• Human/animal tissue