Instruments
The FlexSTAR workflow, which extracts DNA from 0.5-10 ml of sample/protocols (i.e. whole blood), combines our robust and reliable instrumentation with Qiagen’s proven Flexigene chemistry. Our instrument, which is fully automated and easy-to-use with no manual intervention required, can isolate up to 30 samples per run – consistently producing high-quality, high-yield genomic DNA ready for your next application or long-term storage by biorepositories. The FlexSTAR offers a sample tracking and reporting package that utilizes bar code identification of samples to preserve sample integrity.
Principle & Procedure
- Lysis buffer is added to the sample (e.g. blood) and cell nuclei and mitochondria are pelleted by centrifugation
- The pellet is resuspended and incubated in denaturation buffer containing a chaotropic salt and QIAGEN Protease – efficiently removing contaminants (e.g. proteins)
- DNA is precipitated by the addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, dried, and resuspended in hydration buffer (10mM Tris·Cl, pH 8.5)
Instrument Features
- Uses Qiagen’s proven precipitation chemistry for high molecular weight genomic DNA, free of RNA contamination
- Produces high-yield, high-quality DNA ready for immediate use or long-term storage
- Features protocols for 0.5-2 ml, 3-5ml, and 6-10ml of fresh or frozen whole blood
- Includes our Oragene® and Isohelix Saliva protocols allowing labs to process up to 30 4ml saliva tubes at a time
- Process culture cell samples, up to 30 samples of 80 million cells each
- Plus protocols for buffy coats derived from 10ml (30 at a time) or 20ml (15 at a time)
- Runs up to 30 samples in a single batch
- Has an internal barcode scanner for positive sample tracking to verify sample input and DNA output positions
Tracking & Reporting Features
- Compiles run reports and communicates directly to LIMs system
- Creates maps of samples and DNA output positions
- Tracks samples through the extraction process
- Sends emails or text alerts when the run is complete
Sample Type/Protocols
- Cells
- PBMCs
- Oragene DNA saliva
- Fresh/frozen buffy coats
- Buccal swabs
- Human/animal tissue
