Instruments
The FlexSTAR workflow with (+/Plus) Integration is our fully automated solution for genomic DNA extraction and isolation from large volumes of whole blood, buffy coats, and more. Our FlexSTAR+ is the first and only totally automated system that uses Qiagen’s FlexiGene chemistry to isolate genomic DNA free of RNA and other contaminants; it also has the ability to read and record barcodes on primary tubes to preserve sample integrity throughout the process. This proven precipitation chemistry produces pure, high molecular weight gDNA ready for immediate use and long-term storage.
Principle & Procedure
- Lysis buffer is added to the sample type (e.g. blood) and cell nuclei and mitochondria are pelleted by centrifugation
- The pellet is resuspended and incubated in denaturation buffer containing a chaotropic salt and QIAGEN Protease – efficiently removing contaminants (e.g. proteins)
- DNA is precipitated by the addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, dried, and resuspended in hydration buffer (10mM Tris·Cl, pH 8.5)
Instrument Features
- Automates the entire genomic DNA extraction process from primary tubes to DNA storage tubes
- Uses Qiagen’s proven precipitation chemistry for pure genomic DNA, free of RNA contamination
- Produces consistently high-yield, high-quality DNA ready for immediate use or long-term storage
- Includes protocols for 0.5-2ml, 3-5ml, and 6-10ml of fresh or frozen whole blood
- Includes protocols for buffy coats derived from 10 ml (30 at a time) or 20 ml (15 at a time)
- Runs up to 30 samples in a single batch
- Draws samples from a variety of primary tube sizes
- Reads and records barcodes on primary tubes
- Has an internal barcode scanner to verify sample input and DNA output positions
- Provides liquid level detection for accurate pipetting using positive air pressure that senses, measure, and records total volume – allowing for various input volumes
- Includes clot detection that alerts the user before proceeding with sample
- Delivers final elution in SBS-type storage tubes of the user’s choosing
Tracking & Reporting Features
- Compiles run reports and communicates directly to LIMs system
- Creates maps of samples and DNA output positions
- Tracks samples through the extraction process
- Sends emails or text alerts when the run is complete
Sample Type/Protocols
- Cells/PBMCs
- Buccal swabs
- Bone marrow
- Oragene DNA saliva
- Human/animal tissue
- Fresh/frozen buffy coats
Liquid Handling Capabilities
- Plate replication
- Blood aliquot storage
