FlexSTAR+

FlexSTAR+

Our FlexSTAR workflow increases both the quality and productivity of genomic DNA isolation with its (+/Plus) integration, offering simplified, pure gDNA isolation from primary sample tube to DNA storage tube.

  • Load up to 30 samples from a variety of primary tube sizes directly onto the instrument
  • Includes protocols for 0.5 to 10ml whole blood
  • Includes our newly released Oragene® Saliva protocol allowing you to process up to 30, 4ml saliva tubes at a time
  • Plus protocols for buffy coats derived from 10ml (30 at a time) or 20ml (15 at a time)
  • Automated sample transfer from primary tubes with linear and 2D barcode scanning, and LIMS/LIS integration 
  • Additional protocols for cultured cells, packed cells, PBMC, and more

 

Category:

Instruments

The FlexSTAR+ (FlexSTAR workflow with (+/Plus) integration) is our fully automated solution for gDNA extraction and isolation from large volumes of blood, Oragene saliva,  buffy coats, and more. Our FlexSTAR+ is the first and only totally automated system utilizing Qiagen’s FlexiGene chemistry to isolate genomic DNA free of RNA and other contaminants.  The FlexSTAR+ also offers full sample tracking and LIS interfacing.  This proven precipitation chemistry produces pure, high molecular weight gDNA ready for immediate use and long-term storage.

Principle & Procedure
  1. Lysis buffer is added to the sample (e.g. blood) and cell nuclei and mitochondria are pelleted by centrifugation
  2. The pellet is resuspended and incubated in denaturation buffer containing a chaotropic salt and QIAGEN Protease – efficiently removing contaminants (e.g. proteins)
  3. DNA is precipitated by the addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, dried, and resuspended in hydration buffer (10mM Tris·Cl, pH 8.5)
Instrument Features
  • 100% automation – aspirating from primary input tubes to output tubes/storage plate
  • Uses Qiagen’s proven precipitation chemistry for pure genomic DNA, free of RNA contamination
  • Produces high molecular weigh DNA ready for immediate use or long-term storage
  • Blood protocols for processing 0.5 to 10ml from primary tubes
  • New Oragene® Saliva protocol allowing labs to process up to 30, 4ml saliva tubes at a time
  • Additional flexibility for processing buffy coats derived from 10 ml (30 at a time) or 20 ml (15 at a time)
  • Samples from a variety of primary tube sizes and incorporates full sample tracking
  • Integrates full liquid level detection and clot detection for seamless pipetting using positive air pressure that sense, measures, and records the original starting volume in our sample tracking software
  • Flexibility to transfer eluate into a variety of SBS format storage plates
Tracking & Reporting Features
  • Full sample tracking seamlessly communicates with LIS, offering primary tube and eluate tracking
  • Tracks samples through the extraction process easily allowing for a restart in the event of an error
  • Sends emails or text alerts when the run is complete
Sample Type/Protocols
  • Large volume blood
  • Oragene DNA saliva
  • Buffy coats derived from large volumes of blood
  • Cells/PBMCs
  • Bone marrow
  • Human/animal tissue
Liquid Handling Capabilities
  • Plate replication
  • Blood aliquot storage

 

Instrument Dimensions: 63.3″ W x 30″ L x 60.4” H ((890mm W x 715mm D x 1535mm H)

Weight: 1,047lbs (200Kg)

Power:

  • Voltage: Single-phase AC 100-230V
  • Capacity: 0.5kVA, 50/60Hz

Blood Collection Tube Size

  • 2ml φ13 x 75mm (special adapter required)
  • 6ml φ13 x 100mm (special adapter required)
  • 10ml φ16 x 100mm

Bar Code Available For Blood Collection Tube

  • One or two dimensions (maximum of 24 digits; one-byte numeric character only)
    • One dimension: JAN/EAN/UPC, ITF, Codabar, Code39, Code128, EAN128, RSS
    • Two dimensions: DataMatrix (ECC200), PDF417, MaxiCode, QR code, Micro QR, Micro PDF, Composite, AztecCode

Pipette Tip: 1.2ml specially designed tip

  • 96 pieces x 2 plates can be mounted

Liquid Transfer Volume: 100μl-10ml

Dispensing Accuracy: 1ml upon liquid transfer 5% or less (purified water)

Available Storage Tube: 96 x 2 plates

  • 0.75ml, 1ml, 2ml, and 1.4ml matrix tubes of Thermo Fisher are recommended