FlexSTAR

FlexSTAR

Our walk-away FlexSTAR workflow is a cost-effective solution for fully automated genomic DNA extraction and isolation.

  • Isolates DNA from whole blood, buffy coats, tissues, cells, buccal swabs, Oragene saliva, and more
  • Loads up to 30 samples x 0.5-5ml or 15 6-10ml samples/run
  • Includes protocols for buffy coats derived from 10ml (30 at a time) or 20ml (15 at a time)
  • Offers an internal barcode scanner for sample tracking integrity

Instruments

The FlexSTAR workflow, which extracts DNA from 0.5-10 ml of sample/protocols (i.e. whole blood), combines our robust and reliable instrumentation with Qiagen’s proven Flexigene chemistry. Our instrument, which is fully automated and easy-to-use with no manual intervention required, can isolate up to 30 samples per run – consistently producing high-quality, high-yield genomic DNA ready for your next application or long-term storage by biorepositories. The FlexSTAR offers a sample tracking and reporting package that utilizes bar code identification of samples to preserve sample integrity.

Principle & Procedure
  1. Lysis buffer is added to the sample (e.g. blood) and cell nuclei and mitochondria are pelleted by centrifugation
  2. The pellet is resuspended and incubated in denaturation buffer containing a chaotropic salt and QIAGEN Protease – efficiently removing contaminants (e.g. proteins)
  3. DNA is precipitated by the addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, dried, and resuspended in hydration buffer (10mM Tris·Cl, pH 8.5)
Instrument Features
  • Uses Qiagen’s proven precipitation chemistry for high molecular weight genomic DNA, free of RNA contamination
  • Produces high-yield, high-quality DNA ready for immediate use or long-term storage
  • Includes protocols for 0.5-2 ml, 3-5ml, and 6-10ml of fresh or frozen whole blood
  • Includes protocols for buffy coats derived from 10ml (30 at a time) or 20ml (15 at a time)
  • Runs up to 30 samples in a single batch
  • Has an internal barcode scanner for positive sample tracking to verify sample input and DNA output positions
Tracking & Reporting Features
  • Compiles run reports and communicates directly to LIMs system
  • Creates maps of samples and DNA output positions
  • Tracks samples through the extraction process
  • Sends emails or text alerts when the run is complete
Sample Type/Protocols
  • Cells/PBMCs
  • Buccal swabs
  • Bone marrow
  • Oragene DNA saliva
  • Human/animal tissue
  • Fresh/frozen buffy coats

Instrument Dimensions: 28.3”W x 30”L x 60.4”H

Weight: 441lbs

Power:

  • Voltage: Single-phase AC 100-230V
  • Capacity: 0.5kVA, 50/60Hz

Available Storage Tube: 2ml

Learn More About Our Other Instruments